Background: Seungma-Galgeun-Tang (SMGGT), a traditional herbal medicinal formula, has been used to treat\nvarious skin problems such as inflammation and rashes in Korean traditional medicine. In order to clarify the\nscientific evidence for the biological efficacy of SMGGT on the prevention of skin aging and in particular wrinkle\nformation, molecular anti-wrinkle parameters were evaluated in cultured human dermal fibroblasts.\nMethods: Standard SMGGT was prepared from KFDA-certified herbal medicines and the chemical fingerprint of\nSMGGT was verified by HPLC-ESI-MS to insure the quality of SMGGT. To evaluate the inhibitory effects of SMGGT on\nthe synthesis of matrix metalloproteinase-1 (MMP-1) and type-1 procollagen, the content of MMP-1 and type-1\nprocollagen synthesizing enzymes in cultured human dermal fibroblasts were measured using an ELISA kit and\nWestern Blot, respectively.\nResults: The treatment of SMGGT water extract significantly inhibited the production of MMP-1 and promoted\ntype-1 procollagen synthesis concentration dependently.\nConclusions: These results suggest that SMGGT has the potential to prevent wrinkle formation by down-regulating\nMMP-1 and up-regulating type-1 procollagen in human dermal fibroblasts.
Loading....